SMOC1-유래 펩타이드가 골수줄기세포의 조골세포 분화에 미치는 영향
Effect of SMOC1-Derived Peptide on Osteoblast Differentiation of Human Bone Marrow Mesenchymal Stem Cells
최영애, 신홍인, 박의균,
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최영애 ( Choi Young-Ae ) - 경북대학교 치의학전문대학원 구강병리학교실
신홍인 ( Shin Hong-In ) - 경북대학교 치의학전문대학원 구강병리학교실
박의균 ( Park Eui-Kyun ) - 경북대학교 치의학전문대학원 구강병리학교실
KMID : 0829220140380010029
Abstract
Bioactive peptides function effectively with a minimal amount compared to proteins. Recently SPARC related modular calcium binding 1 (SMOC1) has been implicated in regulating osteoblast differentiation and limb and eye development. In this study we synthesized a peptide covering 16 amino acids derived from the extracellular calcium binding (EC) domain of SMOC1, and its effects on proliferation and osteoblast differentiation of human bone marrow mesenchymal stem cells were examined. Treatment of SMOC1 peptide did not modulate proliferation of BMSCs. However, mineralization of BMSCs was significantly increased with a dose dependent manner. Consistently expression of osteoblast differentiation marker genes including type 1 collagen and osteocalcin was also dose dependently increased. Taken together, these results suggest that peptide derived from the EC domain of SMOC1 recapitulates at least partially osteogenic function of SMOC1.
키워드
SMOC1; Bioactive peptide; Osteoblast differentiation; BMSCs
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